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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 12087 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepCMV5
- Backbone size w/o insert (bp) 4600
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameHDM2
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SpeciesH. sapiens (human)
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MutationNuclear-export sequence mutant (NES-): L205A, I208A
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Entrez GeneMDM2 (a.k.a. ACTFS, HDMX, LSKB, hdm2)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ sequencing primer CMV-F (Common Sequencing Primers)
Resource Information
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A portion of this plasmid was derived from a plasmid made byJ. Xiao (Boston University)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pCMV HDM2(NES-) was a gift from Tyler Jacks (Addgene plasmid # 12087 ; http://n2t.net/addgene:12087 ; RRID:Addgene_12087) -
For your References section:
An intact HDM2 RING-finger domain is required for nuclear exclusion of p53. Boyd SD, Tsai KY, Jacks T. Nat Cell Biol. 2000 Sep . 2(9):563-8. 10.1038/35023500 PubMed 10980695