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Addgene

pMT-P4
(Plasmid #120866)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 120866 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    ColE1
  • Vector type
    Synthetic Biology

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Permuteposon P4
  • Species
    Synthetic
  • Insert Size (bp)
    1911
  • GenBank ID
    Permuteposon P4 Permuteposon P4

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

For detailed annotations of the modified R1R2 and R2R1 sites see the Genbank file under Resource Information/Supplemental Documents.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pMT-P4 was a gift from Jonathan Silberg (Addgene plasmid # 120866 ; http://n2t.net/addgene:120866 ; RRID:Addgene_120866)
  • For your References section:

    Overcoming component limitations in synthetic biology through transposon-mediated protein engineering. Atkinson JT, Wu B, Segatori L, Silberg JJ. Methods Enzymol. 2019;621:191-212. doi: 10.1016/bs.mie.2019.02.014. Epub 2019 Apr 2. 10.1016/bs.mie.2019.02.014 PubMed 31128779