pMT-P2
(Plasmid
#120864)
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PurposeEncodes permuteposon P2 for MuA transposase-mediated circular permutation
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 120864 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backboneColE1
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Vector typeSynthetic Biology
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namePermuteposon P2
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Insert Size (bp)1805
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GenBank IDPermuteposon P2
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
For detailed annotations of the modified R1R2 and R2R1 sites see the Genbank file under Resource Information/Supplemental Documents.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pMT-P2 was a gift from Jonathan Silberg (Addgene plasmid # 120864 ; http://n2t.net/addgene:120864 ; RRID:Addgene_120864) -
For your References section:
Overcoming component limitations in synthetic biology through transposon-mediated protein engineering. Atkinson JT, Wu B, Segatori L, Silberg JJ. Methods Enzymol. 2019;621:191-212. doi: 10.1016/bs.mie.2019.02.014. Epub 2019 Apr 2. 10.1016/bs.mie.2019.02.014 PubMed 31128779