pLenti CMVie-IRES-BlastR alt MCS
(Plasmid #120862)

• Purpose: (Empty Backbone) 3rd generation lentiviral expression under a CMVie promoter with Blasticidin selection
• Depositing Lab: Ghassan Mouneimne
• Publication: Puleo et al J Cell Biol. 2019 Oct 8. pii: jcb.201902101. doi: 10.1083/jcb.201902101.

Backbone

• Vector backbone: pCIG3
• Backbone size (bp): 7235
• Modifications to backbone: Replaced GFP with Blasticidin Resistance
• Vector type: Mammalian Expression, Lentiviral
• Promoter: CMV immediate early
• Selectable markers: Blasticidin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 30°C
• Growth Strain(s): NEB Stable
• Copy number: Low Copy

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ sequencing primer: CMV Fwd
• 3′ sequencing primer: IRES Rev

Resource Information

• Supplemental Documents:
  • pCIB (CMV-IRES-BLAST, alt MCS).gb
• A portion of this plasmid was derived from a plasmid made by: Modification of empty lentiviral vector pCIG3 (Addgene 78264), Goodrum Lab
• Articles Citing this Plasmid:
  • 2 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Please note that though there are some discrepancies between the backbone sequence from the depositing lab and Addgene's quality control sequence, this plasmid should function as described.