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Addgene

NK695
(Plasmid #120854)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 120854 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    NK676
  • Vector type
    Choanoflagellate expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    pac-P2A-Jumblelw1-mTFP
  • Alt name
    pEFl5'-Actin3'::pac-P2A-Jumblelw1-mTFP
  • Mutation
    Jumblelw1 allele: T to C at nt 1109 causes L305P mutation
  • GenBank ID
    XM_004998928

Cloning Information

  • Cloning method Gibson Cloning

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    NK695 was a gift from Nicole King (Addgene plasmid # 120854 ; http://n2t.net/addgene:120854 ; RRID:Addgene_120854)