NK691
(Plasmid
#120850)
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PurposeReporter gene expression in S. rosetta
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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 120850 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepUC19
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Vector typeChoanoflagellate expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameJumblelw1-mWasabi
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Alt namepEFl5'-Actin3'::Jumblelw1-mWasabi
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MutationJumblelw1 allele: T to C at nt 1109 causes L305P mutation
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GenBank IDXM_004998928
Cloning Information
- Cloning method Gibson Cloning
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
NK691 was a gift from Nicole King (Addgene plasmid # 120850 ; http://n2t.net/addgene:120850 ; RRID:Addgene_120850)