pSicoR PGK puro
(Plasmid #12084)

• Purpose: (Empty Backbone)
• Depositing Lab: Tyler Jacks
• Publication: Ventura et al Proc Natl Acad Sci U S A. 2004 Jul 13. 101(28):10380-5.

Backbone

• Vector backbone: pSicoR PGK puro
• Backbone size (bp): 7700
• Modifications to backbone: Puromycin is expressed from this plasmid as a selectable marker. Puromycin and the shRNA oligo are flanked by LoxP sites. Cre causes both to be recombined out of the construct, turning off shRNA expression.
• Vector type: Mammalian Expression, Lentiviral, RNAi, Cre/Lox
• Selectable markers: Puromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: None

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: HpaI (not destroyed)
• 3′ cloning site: XhoI (not destroyed)
• 5′ sequencing primer: mU6-F

Resource Information

• Articles Citing this Plasmid:
  • 10 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

pSicoR PGK Puro allows for conditional (Cre-Lox), stable expression of shRNAs for RNA interference in cells and transgenic mice. Addition of Cre TURNS OFF shRNA expression. See pSicoR and pSico PGK Puro for more information.

The shRNA coding oligos have to be cloned into the HpaI and XhoI restriction sites. Oligo design information can be found on the author's map for pSico and on the Jacks Lab website http://web.mit.edu/jacks-lab/protocols/pSico.html