pDSW1728
(Plasmid
#120812)
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PurposeE. coli-C. difficile shuttle vector: tetracycline-inducible promoter (Ptet) driving expression of red fluorescent protein (mCherryOpt)
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Depositing Labs
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 120812 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepRFP185
- Backbone size w/o insert (bp) 6400
- Total vector size (bp) 7911
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Modifications to backbonePtet::mCherryOpt
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Chloramphenicol, 25 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Growth instructionsLow copy in C. difficile following conjugation and selected with thiamphenicol
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namered fluorescent protein (mCherry), codon-optimized for C. difficile
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Alt namemCherryOpt
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SpeciesSynthetic
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Insert Size (bp)1529
- Promoter Ptet
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site SacI (not destroyed)
- 3′ cloning site BamHI (not destroyed)
- 5′ sequencing primer unknown
- 3′ sequencing primer unknown (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pDSW1728 was a gift from Craig Ellermeier & David S. Weiss (Addgene plasmid # 120812 ; http://n2t.net/addgene:120812 ; RRID:Addgene_120812) -
For your References section:
Use of mCherry Red fluorescent protein for studies of protein localization and gene expression in Clostridium difficile. Ransom EM, Ellermeier CD, Weiss DS. Appl Environ Microbiol. 2015 Mar;81(5):1652-60. doi: 10.1128/AEM.03446-14. Epub 2014 Dec 19. 10.1128/AEM.03446-14 PubMed 25527559