S10A mutant of H3S10ph biosensor
(Plasmid #120810)

• Purpose: FRET biosensor. Eliminating the H3 Serine 10 phosphorylation site in H3S10ph biosensor to abolish the detection capability
• Depositing Lab: Yingxiao Wang
• Publication: Peng et al Proc Natl Acad Sci U S A. 2018 Nov 26. pii: 1811818115. doi: 10.1073/pnas.1811818115.

Backbone

• Vector backbone: pcDNA3.1
• Backbone manufacturer: Invitrogen
• Backbone size w/o insert (bp): 5368
• Total vector size (bp): 7786
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Mouse histone H3-CFP-FHA2-histone H3 peptide (1-14aa) S10A-YFP
• Species: M. musculus (mouse), Synthetic
• Insert Size (bp): 2418
• Mutation: Serine 10 is mutated to Alanine, and Threonine 3, Threonine 6, and Threonine 11 are mutated to Alanine on histone H3 peptide (1-14aa)
• Promoter: CMV

Cloning Information

• Cloning method: Gibson Cloning
• 5′ sequencing primer: T7
• 3′ sequencing primer: BGHR

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry