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pET-21a_2xNLS_FnCpf1_MBP_protein_expression
(Plasmid #120801)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 120801 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pET-21 a
  • Backbone size w/o insert (bp) 5400
  • Total vector size (bp) 9705
  • Vector type
    Bacterial Expression, CRISPR

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    2xNLS_FnCpf1
  • Species
    Synthetic; Francisella novicida
  • Insert Size (bp)
    3778
  • Promoter T7
  • Tags / Fusion Proteins
    • NLS (C terminal on insert)
    • NLS (C terminal on insert)
    • MBP (C terminal on insert)

Cloning Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pET-21a_2xNLS_FnCpf1_MBP_protein_expression was a gift from Scot Wolfe (Addgene plasmid # 120801 ; http://n2t.net/addgene:120801 ; RRID:Addgene_120801)

  • For your References section:

    Enhanced Cas12a editing in mammalian cells and zebrafish. Liu P, Luk K, Shin M, Idrizi F, Kwok S, Roscoe B, Mintzer E, Suresh S, Morrison K, Frazao JB, Bolukbasi MF, Ponnienselvan K, Luban J, Zhu LJ, Lawson ND, Wolfe SA. Nucleic Acids Res. 2019 Mar 20. pii: 5403491. doi: 10.1093/nar/gkz184. 10.1093/nar/gkz184 PubMed 30892626
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