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Addgene

NM R2E2 GFP
(Plasmid #1208)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 1208 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pRS316
  • Backbone size w/o insert (bp) 4847
  • Vector type
    Yeast Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    sup35 NM domain
  • Alt name
    sup35
  • Species
    S. cerevisiae (budding yeast)
  • Insert Size (bp)
    1990
  • Mutation
    2 additional copies of second oligopeptide repeat in sup35 N domain
  • GenBank ID
    AY553990
  • Entrez Gene
    SUP35 (a.k.a. YDR172W, GST1, PNM2, SAL3, SUF12, SUP2, SUP36)
  • Tag / Fusion Protein
    • GFP (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site GFP cloned into CUP1-NM with SacI (not destroyed)
  • 5′ sequencing primer n/a
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    NM R2E2 GFP was a gift from Susan Lindquist (Addgene plasmid # 1208 ; http://n2t.net/addgene:1208 ; RRID:Addgene_1208)

  • For your References section:

    Oligopeptide-repeat expansions modulate 'protein-only' inheritance in yeast. Liu JJ, Lindquist S. Nature 1999 Aug 5;400(6744):573-6. 10.1038/23048 PubMed 10448860
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