pAP114
(Plasmid #120799)

• Purpose: E. coli-C. difficile shuttle vector for xylose-inducible expression in C. difficile; Pxyl driving expression of red fluorescent protein (mCherryOpt)
• Depositing Labs: Craig Ellermeier, David S. Weiss
• Publication: Muh et al J Bacteriol. 2019 Feb 11. pii: JB.00711-18. doi: 10.1128/JB.00711-18.

Backbone

• Vector backbone: pDSW1728
• Backbone size w/o insert (bp): 6400
• Total vector size (bp): 8513
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Chloramphenicol, 25 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Growth instructions: Low copy in C. difficile following conjugation and selected with thiamphenicol
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Pxyl::mCherryOpt
• Species: C. difficile
• Insert Size (bp): 2093
• Mutation: 1.4 kb xylR-Pxyl DNA fragment from C. difficile R20291 controls expression of mCherryOpt
• Promoter: Pxyl

Cloning Information

• Cloning method: Gibson Cloning
• 5′ sequencing primer: unknown
• 3′ sequencing primer: unknown

Resource Information

• Supplemental Documents:
  • pAP114.gb
• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry