pIS0 - HOXA7 3'UTR
(Plasmid #12079)

• Depositing Lab: David Bartel
• Publication: Yekta et al Science. 2004 Apr 23. 304(5670):594-6.

Backbone

• Vector backbone: pIS0
• Backbone manufacturer: Bartel Lab
• Backbone size w/o insert (bp): 5260
• Vector type: Mammalian Expression, Luciferase

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: HOXA7 3'UTR
• Alt name: HOXA7
• Species: H. sapiens (human)
• Insert Size (bp): 230
• GenBank ID: NM_006896
• Entrez Gene: HOXA7 (a.k.a. ANTP, HOX1, HOX1A, HOX1.1)
• Tag / Fusion Protein:
  • luciferase (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: SacI (not destroyed)
• 3′ cloning site: XbaI (not destroyed)
• 5′ sequencing primer: n/a
• 3′ sequencing primer: EBV rev primer

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Luciferase Limited Use Label License
• Industry Terms:
  • Not Available to Industry

Depositor Comments

HOXA7 3'UTR cloned downstream of the firefly luciferase open reading frame. In the author's map, seed matches are shown in blue.