GGE254
(Plasmid
#120789)
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PurposePart of GoldenGate Yarrowia lipolytica toolkit for assembling an expression plasmid for Y. lipolytic yeast. Sequence for homologous integration into Y. lipolytica genome. Linearization of cassette by NotI restriction enzyme after the assembly
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 120789 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepCR4Blunt-TOPO
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Vector typeCloning/assembly plasmid
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameInsDown_Lip-NotI
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Insert Size (bp)1008
Cloning Information
- Cloning method TOPO Cloning
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
GGE254 was a gift from Tristan Rossignol (Addgene plasmid # 120789 ; http://n2t.net/addgene:120789 ; RRID:Addgene_120789) -
For your References section:
A modular Golden Gate toolkit for Yarrowia lipolytica synthetic biology. Larroude M, Park YK, Soudier P, Kubiak M, Nicaud JM, Rossignol T. Microb Biotechnol. 2019 May 31. doi: 10.1111/1751-7915.13427. 10.1111/1751-7915.13427 PubMed 31148366
Included in kit:
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