pIS0 - HOXB8 3'UTR
(Plasmid
#12076)
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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 12076 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepIS0
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Backbone manufacturerBartel Lab
- Backbone size w/o insert (bp) 5260
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Vector typeMammalian Expression, Luciferase
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameHOXB8 3'UTR
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Alt nameHOXB8
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SpeciesH. sapiens (human)
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Insert Size (bp)22
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Entrez GeneHOXB8 (a.k.a. HOX2, HOX2D, Hox-2.4)
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Tag
/ Fusion Protein
- luciferase (N terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site SacI (not destroyed)
- 3′ cloning site XbaI (not destroyed)
- 5′ sequencing primer n/a
- 3′ sequencing primer EBV rev primer (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
HOXB8 3'UTR cloned downstream of the firefly luciferase open reading frame.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pIS0 - HOXB8 3'UTR was a gift from David Bartel (Addgene plasmid # 12076 ; http://n2t.net/addgene:12076 ; RRID:Addgene_12076) -
For your References section:
MicroRNA-directed cleavage of HOXB8 mRNA. Yekta S, Shih IH, Bartel DP. Science. 2004 Apr 23. 304(5670):594-6. 10.1126/science.1097434 PubMed 15105502