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35S:5mARF17 pLBR19
(Plasmid #12073)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 12073 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pLBR19
  • Backbone manufacturer
    (pLBR19 is derived from pUC19, but contains the CaMV 35S promote
  • Backbone size w/o insert (bp) 5500
  • Vector type
    Plant Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    5mARF17
  • Alt name
    auxin response transcription factor 17
  • Alt name
    ARF17
  • Species
    A. thaliana (mustard weed)
  • Insert Size (bp)
    3300
  • Mutation
    miR160-resistant version of ARF17 has five silent mutations within the miR160-complementary domain of an ARF17 genomic clone, thereby increasing the number of mismatches between miR160 and the ARF17 mRNA from one in wild type to six.
  • Entrez Gene
    ARF17 (a.k.a. AT1G77850, F28K19.6, F28K19_6, auxin response factor 17)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer 35Spromoter (5'-CTA TCC TTC GCA AGA CCC TTC-3')
  • 3′ sequencing primer CaMV term rev (5'-TTG ATA GAA GTA TTT TAC AAA TAC-3')
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

To generate 35S:5mARF17, a 3.3 kb EcoR1-BamH1 fragment of the 6.6 kb 5mARF17 pBluescript clone was subcloned into pLBR19 downstream from the duplicated enhancer of CaMV 35S RNA promoter. See "sequence" for full insert sequence.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    35S:5mARF17 pLBR19 was a gift from David Bartel (Addgene plasmid # 12073 ; http://n2t.net/addgene:12073 ; RRID:Addgene_12073)

  • For your References section:

    MicroRNA-directed regulation of Arabidopsis AUXIN RESPONSE FACTOR17 is essential for proper development and modulates expression of early auxin response genes. Mallory AC, Bartel DP, Bartel B. Plant Cell. 2005 May . 17(5):1360-75. 10.1105/tpc.105.031716 PubMed 15829600
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