pcDNA-DDX11-Flag-KAK
(Plasmid #120729)

• Purpose: Expression of a Flag-tagged version of DDX11 KAK mutant in mammalian cells
• Depositing Lab: Francesca Pisani
• Publication: Cortone et al PLoS Genet. 2018 Oct 10;14(10):e1007622. doi: 10.1371/journal.pgen.1007622. eCollection 2018 Oct.

Backbone

• Vector backbone: pcDNA 3
• Backbone manufacturer: Invitrogen
• Backbone size w/o insert (bp): 5446
• Total vector size (bp): 8230
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: DDX11 KAK mutant
• Alt name: Chlr1 KAK
• Species: H. sapiens (human)
• Insert Size (bp): 2784
• Mutation: Substitution of E201, Y202 and E203 with K, A and K
• Entrez Gene: DDX11 (a.k.a. CHL1, CHLR1, KRG2, WABS)
• Promoter: CMV
• Tag / Fusion Protein:
  • 3x FLAG (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: HindIII (not destroyed)
• 3′ cloning site: XhoI (not destroyed)
• 5′ sequencing primer: CMV-F

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Mutations were created by a PCR based method.