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Addgene

pLV-shGrm2-2344
(Plasmid #120725)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 120725 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pLL3.7
  • Backbone manufacturer
    MIT
  • Backbone size w/o insert (bp) 7649
  • Total vector size (bp) 7700
  • Vector type
    Mammalian Expression, Lentiviral, RNAi

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    30°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Grm2
  • gRNA/shRNA sequence
    gtggtgacattgcgctgta
  • Species
    R. norvegicus (rat)
  • Entrez Gene
    Grm2
  • Promoter mouse U6
  • Tag / Fusion Protein
    • GFP

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site HpaI (destroyed during cloning)
  • 3′ cloning site XhoI (not destroyed)
  • 5′ sequencing primer U6
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

This plasmid was produced as part of the NIAAA-funded INIA Consortium.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pLV-shGrm2-2344 was a gift from Amy Lasek (Addgene plasmid # 120725 ; http://n2t.net/addgene:120725 ; RRID:Addgene_120725)

  • For your References section:

    Reduced Levels of mGlu2 Receptors within the Prelimbic Cortex Are Not Associated with Elevated Glutamate Transmission or High Alcohol Drinking. Ding ZM, Ingraham CM, Hauser SR, Lasek AW, Bell RL, McBride WJ. Alcohol Clin Exp Res. 2017 Nov;41(11):1896-1906. doi: 10.1111/acer.13488. Epub 2017 Oct 3. 10.1111/acer.13488 PubMed 28858384
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