pLV-shKcnk13
(Plasmid #120721)

• Purpose: Expresses GFP and an shRNA targeting Kcnk13 (in pLL3.7)
• Depositing Lab: Amy Lasek
• Publication: You et al Neuropharmacology. 2018 Oct 14;144:29-36. doi: 10.1016/j.neuropharm.2018.10.008.

Backbone

• Vector backbone: pLL3.7
• Backbone manufacturer: MIT
• Backbone size w/o insert (bp): 7649
• Total vector size (bp): 7700
• Vector type: Mammalian Expression, Lentiviral, RNAi

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 30°C
• Growth Strain(s): NEB Stable
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Kcnk13
• gRNA/shRNA sequence: GGAATAAGACACTGTTACACC
• Species: M. musculus (mouse)
• Entrez Gene: Kcnk13 (a.k.a. BB085247, F730021E22Rik, Gm1570, Gm1685)
• Promoter: mouse U6
• Tag / Fusion Protein:
  • GFP

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: HpaI (destroyed during cloning)
• 3′ cloning site: XhoI (not destroyed)
• 5′ sequencing primer: U6

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry