pLEX-Frb:Akt2-IRES-Hygromycin
(Plasmid #120713)

• Purpose: Expresses Frb:Akt2 fusion protein in mammalian cells & for virus production
• Depositing Lab: Paul Khavari
• Publication: Kovalski et al Mol Cell. 2019 Jan 4. pii: S1097-2765(18)31031-1. doi: 10.1016/j.molcel.2018.12.001.

Backbone

• Vector backbone: pLEX
• Backbone manufacturer: Thermo Scientific
• Backbone size w/o insert (bp): 11000
• Total vector size (bp): 12000
• Modifications to backbone: Puromycin resistance cassette swapped for hygromycin resistance.
• Vector type: Mammalian Expression, Lentiviral
• Selectable markers: Hygromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: AKT2
• Species: H. sapiens (human)
• Insert Size (bp): 1739
• Entrez Gene: AKT2 (a.k.a. HIHGHH, PKBB, PKBBETA, PRKBB, RAC-BETA)
• Promoter: CMV
• Tag / Fusion Protein:
  • FRB(T2098L) (N terminal on insert)

Cloning Information

• Cloning method: Ligation Independent Cloning
• 5′ sequencing primer: CACCAAAATCAACGGGACTT
• 3′ sequencing primer: ATATAGACAAACGCACACCGGCCT

Resource Information

• A portion of this plasmid was derived from a plasmid made by: Frb:Akt2 insert was cloned out of Addgene plasmid #72899.

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry