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Addgene

ARF17 pGreenII0229
(Plasmid #12070)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 12070 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pGreenII0229
  • Backbone size w/o insert (bp) 5000
  • Vector type
    Plant Expression ; plant transformation via Agrobacterium
  • Selectable markers
    Basta

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    ARF17
  • Alt name
    auxin response transcription factor 17
  • Species
    A. thaliana (mustard weed)
  • Insert Size (bp)
    6600
  • Entrez Gene
    ARF17 (a.k.a. AT1G77850, F28K19.6, F28K19_6, auxin response factor 17)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site SpeI (destroyed during cloning)
  • 5′ sequencing primer M13 forward 20
  • 3′ sequencing primer M13 reverse
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

See "sequence" for full insert sequence. See article for cloning details.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    ARF17 pGreenII0229 was a gift from David Bartel (Addgene plasmid # 12070 ; http://n2t.net/addgene:12070 ; RRID:Addgene_12070)

  • For your References section:

    MicroRNA-directed regulation of Arabidopsis AUXIN RESPONSE FACTOR17 is essential for proper development and modulates expression of early auxin response genes. Mallory AC, Bartel DP, Bartel B. Plant Cell. 2005 May . 17(5):1360-75. 10.1105/tpc.105.031716 PubMed 15829600
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