Smp_006510-Cd4-bio-His
(Plasmid
#120673)
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PurposeExpression of recombinant S. mansoni protein
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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 120673 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepTT3
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Backbone manufacturerYves Durocher, NRC
- Backbone size w/o insert (bp) 6686
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameSmp_006510
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SpeciesSchistosoma mansoni
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Insert Size (bp)718
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Entrez GeneSmp_006510 (a.k.a. Smp_006510)
- Promoter CMV
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Tag
/ Fusion Protein
- ratCd4(d3+4)-bio-6His (C terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site NotI (not destroyed)
- 3′ cloning site AscI (not destroyed)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
Smp_006510-Cd4-bio-His was a gift from Gavin Wright (Addgene plasmid # 120673 ; http://n2t.net/addgene:120673 ; RRID:Addgene_120673) -
For your References section:
A library of cell-surface and secreted proteins from Schistosoma mansoni identifies early serological markers of infection. Cécile Crosnier, Anna V. Protasio, Gabriel Rinaldi, Shona Wilson, Matthew Berriman, Gavin J. Wright. bioRXiv 10.1101/593616