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5mCUC1 pBluescript
(Plasmid #12065)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 12065 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pBluescript II SK+
  • Backbone manufacturer
    Stratagene
  • Backbone size w/o insert (bp) 3000
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    CUC1 mutant
  • Alt name
    CUC1
  • Species
    A. thaliana (mustard weed)
  • Insert Size (bp)
    3700
  • Mutation
    miR-resistant CUC1. Made by introducing five silent mutations within the miR164-complementarity domain of a CUC1 genomic clone.
  • Entrez Gene
    CUC1 (a.k.a. AT3G15170, ANAC054, ATNAC1, Arabidopsis NAC domain containing protein 54, CUP-SHAPED COTYLEDON1)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer T7
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

The CUC1 plasmid (addgene # 12064) was used as a starting material. The sequence of the primer pair used for CUC1 mutagenesis is 5mCUC1 forward 5'-CCGATCATCAATACCTTTGCGACGGAGCATGTTTCATGTTTTT CAAATAACTCTGCTGCTCATACCG-3' and 5mCUC1 reverse 5'-CGGTATGAGCAGCAGAGTTATTTGAAAAACATGAAACATGCTCCGTCGCAAAGGTATTGATGATCGG-3'. See "sequence" for the full insert sequence. See article for cloning details.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    5mCUC1 pBluescript was a gift from David Bartel (Addgene plasmid # 12065 ; http://n2t.net/addgene:12065 ; RRID:Addgene_12065)

  • For your References section:

    MicroRNA regulation of NAC-domain targets is required for proper formation and separation of adjacent embryonic, vegetative, and floral organs. Mallory AC, Dugas DV, Bartel DP, Bartel B. Curr Biol. 2004 Jun 22. 14(12):1035-46. 10.1016/j.cub.2004.06.022 PubMed 15202996
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