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Smp_178740-Cd4-bio-His
(Plasmid #120646)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 120646 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pTT3
  • Backbone manufacturer
    Yves Durocher, NRC
  • Backbone size w/o insert (bp) 6686
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Smp_178740
  • Species
    Schistosoma mansoni
  • Insert Size (bp)
    1510
  • Entrez Gene
    Smp_178740 (a.k.a. Smp_178740)
  • Promoter CMV
  • Tag / Fusion Protein
    • ratCd4(d3+4)-bio-6His (C terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NotI (not destroyed)
  • 3′ cloning site AscI (not destroyed)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Smp_178740-Cd4-bio-His was a gift from Gavin Wright (Addgene plasmid # 120646 ; http://n2t.net/addgene:120646 ; RRID:Addgene_120646)

  • For your References section:

    A library of cell-surface and secreted proteins from Schistosoma mansoni identifies early serological markers of infection. Cécile Crosnier, Anna V. Protasio, Gabriel Rinaldi, Shona Wilson, Matthew Berriman, Gavin J. Wright. bioRXiv 10.1101/593616
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