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Addgene

CUC1 pBluescript
(Plasmid #12064)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 12064 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pBluescript II SK+
  • Backbone manufacturer
    Stratagene
  • Backbone size w/o insert (bp) 3000
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    CUC1
  • Species
    A. thaliana (mustard weed)
  • Insert Size (bp)
    3700
  • Entrez Gene
    CUC1 (a.k.a. AT3G15170, ANAC054, ATNAC1, Arabidopsis NAC domain containing protein 54, CUP-SHAPED COTYLEDON1)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer T7
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

The genomic sequence of CUC1 (At3g15170), including 1.4 kilobases (kb) and 0.7 kb of putative 5' and 3' regulatory sequences, respectively, was cloned as an 3.7 kb BamH1-EcoR1 fragment into pBluescriptIISK+ (Stratagene) from the bacterial artificial chromosome F4B12. See "sequence" for full insert sequence.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    CUC1 pBluescript was a gift from David Bartel (Addgene plasmid # 12064 ; http://n2t.net/addgene:12064 ; RRID:Addgene_12064)

  • For your References section:

    MicroRNA regulation of NAC-domain targets is required for proper formation and separation of adjacent embryonic, vegetative, and floral organs. Mallory AC, Dugas DV, Bartel DP, Bartel B. Curr Biol. 2004 Jun 22. 14(12):1035-46. 10.1016/j.cub.2004.06.022 PubMed 15202996
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