EF1a-Cas9-2XNES-2ERT2-GCN4-SV40-Zeo
(Plasmid #120555)

• Purpose: Express Cas9, 2xNES, 2 tandem ERT2 and GCN4 in mammalian cells
• Depositing Lab: Yu Wang
• Publication: Lu et al Nucleic Acids Res. 2018 Mar 16;46(5):e25. doi: 10.1093/nar/gkx1222.

Backbone

• Vector backbone: pRRL.sin-18.ppy.
• Vector type: Mammalian Expression, Lentiviral
• Selectable markers: Zeocin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Cas9, NES, ERT2 and GCN4
• Species: Synthetic

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: Unknown (unknown if destroyed)
• 3′ cloning site: Unknown (unknown if destroyed)
• 5′ sequencing primer: n/a

Resource Information

• Supplemental Documents:
  • hPGK-Cas9-2XNES-2ERT2-GCN4-SV40-Zeo.gb
• A portion of this plasmid was derived from a plasmid made by: Cas9 was cloned from Addgene plasmid #42230; ERT2 was cloned from the pAd-CreER plasmid (a gift from T. C. He’s lab, Chicago University); NES and GCN4 sequences were synthesized

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry