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Addgene

ScFv-2ERT2-PH
(Plasmid #120554)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 120554 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pRRL.sin-18.ppy.
  • Vector type
    Mammalian Expression, Lentiviral
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    scFvGCN4, sfGFP, GB1, ERT2, P65 and HSF1
  • Species
    Synthetic
  • Promoter hPGK

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Unknown (unknown if destroyed)
  • 3′ cloning site Unknown (unknown if destroyed)
  • 5′ sequencing primer hPGK-F
    • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    ERT2 was cloned from the pAd-CreER plasmid (a gift from T. C. He’s lab, Chicago University); P65 was synthesized according to the sequences reported by Chavez et al; HSF1 was cloned from Addgene plasmid # 61426; scFvGCN4, sfGFP and GB1 were synthesized according to the sequences reported by Tanenbaum et. Al.

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please note that some discrepancies were found between Addgene's quality control result and the depositor's Genbank sequence. The depositor noted that these discrepancies do NOT affect plasmid function.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    ScFv-2ERT2-PH was a gift from Yu Wang (Addgene plasmid # 120554)

  • For your References section:

    Multimode drug inducible CRISPR/Cas9 devices for transcriptional activation and genome editing. Lu J, Zhao C, Zhao Y, Zhang J, Zhang Y, Chen L, Han Q, Ying Y, Peng S, Ai R, Wang Y. Nucleic Acids Res. 2018 Mar 16;46(5):e25. doi: 10.1093/nar/gkx1222. 10.1093/nar/gkx1222 PubMed 29237052
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