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Addgene

EF1a-3NLS-NGN2TALE-GCN4
(Plasmid #120547)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 120547 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pRRL.sin-18.ppy.
  • Vector type
    Mammalian Expression, Lentiviral
  • Selectable markers
    Zeocin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    NLS, NGN2 TALE, GCN4
  • Species
    Synthetic
  • Entrez Gene
    NEUROG2 (a.k.a. Atoh4, Math4A, NGN2, bHLHa8, ngn-2)
  • Promoter EF1α

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Unknown (unknown if destroyed)
  • 3′ cloning site Unknown (unknown if destroyed)
  • 5′ sequencing primer n/a
    • (Common Sequencing Primers)

Resource Information

  • Supplemental Documents
  • A portion of this plasmid was derived from a plasmid made by
    NLS and NGN2 TALE was cloned from addgene plasmid #47458; GCN4 was synthesized according to the sequences reported by Tanenbaum et. Al.

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please note that some discrepancies were found between Addgene's quality control result and the depositor's full plasmid sequence. The depositor noted that these discrepancies do NOT affect plasmid function.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    EF1a-3NLS-NGN2TALE-GCN4 was a gift from Yu Wang (Addgene plasmid # 120547 ; http://n2t.net/addgene:120547 ; RRID:Addgene_120547)

  • For your References section:

    Multiple Chemical Inducible Tal Effectors for Genome Editing and Transcription Activation. Zhao C, Zhang Y, Zhao Y, Ying Y, Ai R, Zhang J, Wang Y. ACS Chem Biol. 2018 Mar 16;13(3):609-617. doi: 10.1021/acschembio.7b00606. Epub 2018 Jan 8. 10.1021/acschembio.7b00606 PubMed 29308880
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