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Addgene

EF1a-3NLS-Klf4 TALE-GCN4
(Plasmid #120546)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 120546 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pRRL.sin-18.ppy.
  • Vector type
    Mammalian Expression, Lentiviral
  • Selectable markers
    Zeocin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    NLS, Klf4 TALE, GCN4
  • Species
    Synthetic
  • Entrez Gene
    KLF4 (a.k.a. EZF, GKLF)
  • Promoter EF1α

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Unknown (unknown if destroyed)
  • 3′ cloning site Unknown (unknown if destroyed)
  • 5′ sequencing primer n/a
    • (Common Sequencing Primers)

Resource Information

  • Supplemental Documents
  • A portion of this plasmid was derived from a plasmid made by
    NLS and Klf4 TALE was cloned from Addgene plasmid #35389; GCN4 was synthesized according to the sequences reported by Tanenbaum et. Al.

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    EF1a-3NLS-Klf4 TALE-GCN4 was a gift from Yu Wang (Addgene plasmid # 120546 ; http://n2t.net/addgene:120546 ; RRID:Addgene_120546)

  • For your References section:

    Multiple Chemical Inducible Tal Effectors for Genome Editing and Transcription Activation. Zhao C, Zhang Y, Zhao Y, Ying Y, Ai R, Zhang J, Wang Y. ACS Chem Biol. 2018 Mar 16;13(3):609-617. doi: 10.1021/acschembio.7b00606. Epub 2018 Jan 8. 10.1021/acschembio.7b00606 PubMed 29308880
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