dFP-mini
(Plasmid #120543)

• Purpose: Expresses dFP biliverdin-binding fluorescence-activating mini 10 KDa protein in bacteria.
• Depositing Lab: Brian Chow
• Publication: Sheehan et al Biochemistry. 2018 Nov 28. doi: 10.1021/acs.biochem.8b01076.

Backbone

• Vector backbone: pET15b
• Backbone manufacturer: Genscript
• Backbone size w/o insert (bp): 5645
• Total vector size (bp): 5999
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: de novo fluorescence-activating protein mini (dFP-mini)
• Alt name: dFP-mini
• Alt name: mini
• Species: Synthetic
• Insert Size (bp): 282
• Promoter: T7
• Tag / Fusion Protein:
  • 6xHis - TEV cleavage (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NcoI, BamHI, NheI (not destroyed)
• 3′ cloning site: EcoRI, XhoI (not destroyed)
• 5′ sequencing primer: T7
• 3′ sequencing primer: T7 Reverse

Resource Information

• Supplemental Documents:
  • dFP-mini - T7 - pET15b.gb

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry