dFP
(Plasmid #120542)

• Purpose: Expresses dFP biliverdin-binding fluorescence-activating full length protein in bacteria.
• Depositing Lab: Brian Chow
• Publication: Sheehan et al Biochemistry. 2018 Nov 28. doi: 10.1021/acs.biochem.8b01076.

Backbone

• Vector backbone: pET15b
• Backbone manufacturer: Genscript
• Backbone size w/o insert (bp): 5632
• Total vector size (bp): 6122
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: de novo fluorescence-activating protein (dFP)
• Alt name: dFP-mini
• Alt name: mini
• Species: Synthetic
• Insert Size (bp): 384
• Promoter: T7
• Tag / Fusion Protein:
  • 6xHis - TEV cleavage (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamHI, NcoI (not destroyed)
• 3′ cloning site: AgeI, XhoI (not destroyed)
• 5′ sequencing primer: T7
• 3′ sequencing primer: T7 Reverse

Resource Information

• Supplemental Documents:
  • dFP-T7-pET15b.gb

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry