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Addgene

pEF1-MH.Bl-mDcrSOM
(Plasmid #120540)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 120540 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pEF1alpha.Blast
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    30°C
  • Growth Strain(s)
    DH5alpha
  • Growth instructions
    Pick small colonies as larger colonies tend to be incorrect.
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Dicer
  • Species
    M. musculus (mouse)
  • Entrez Gene
    Dicer1 (a.k.a. 1110006F08Rik, D12Ertd7e, aviD, mKIAA0928)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Unknown (unknown if destroyed)
  • 3′ cloning site Unknown (unknown if destroyed)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pEF1-MH.Bl-mDcrSOM was a gift from Petr Svoboda (Addgene plasmid # 120540 ; http://n2t.net/addgene:120540 ; RRID:Addgene_120540)