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EF1a_ASCL1_P2A_Hygro_Barcode
(Plasmid #120427)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 120427 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    Unknown
  • Backbone size w/o insert (bp) 9514
  • Vector type
    Lentiviral
  • Selectable markers
    Hygromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    Stbl3
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    ASCL1
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    708
  • Entrez Gene
    ASCL1 (a.k.a. ASH1, HASH1, MASH1, bHLHa46)
  • Promoter EF1a

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

5' cloning site: BamHI (not destroyed), 3' cloning site: BamHI (not destroyed).

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    EF1a_ASCL1_P2A_Hygro_Barcode was a gift from Prashant Mali (Addgene plasmid # 120427 ; http://n2t.net/addgene:120427 ; RRID:Addgene_120427)

  • For your References section:

    Mapping Cellular Reprogramming via Pooled Overexpression Screens with Paired Fitness and Single-Cell RNA-Sequencing Readout. Parekh U, Wu Y, Zhao D, Worlikar A, Shah N, Zhang K, Mali P. Cell Syst. 2018 Nov 12. pii: S2405-4712(18)30433-2. doi: 10.1016/j.cels.2018.10.008. 10.1016/j.cels.2018.10.008 PubMed 30448000
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