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Addgene

pcDNA3 - hSRSF6
(Plasmid #120419)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 120419 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pcDNA3
  • Backbone size w/o insert (bp) 5395
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    SRSF6
  • Alt name
    SRP55, B52
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1041
  • Entrez Gene
    SRSF6 (a.k.a. B52, HEL-S-91, SFRS6, SRP55)
  • Promoter CMV immediate/early

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site NotI (not destroyed)
  • 5′ sequencing primer unknown
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcDNA3 - hSRSF6 was a gift from Gillian Bates & Andreas Neueder (Addgene plasmid # 120419 ; http://n2t.net/addgene:120419 ; RRID:Addgene_120419)

  • For your References section:

    Regulatory mechanisms of incomplete huntingtin mRNA splicing. Neueder A, Dumas AA, Benjamin AC, Bates GP. Nat Commun. 2018 Sep 27;9(1):3955. doi: 10.1038/s41467-018-06281-3. 10.1038/s41467-018-06281-3 [pii] PubMed 30262848
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