Lyn-R-GECO1
(Plasmid
#120410)
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PurposeExpresses a fusion between myristoylation signal peptide and R-GECO1. This construct targets R-GECO1 to the plasma membrane.
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 120410 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backboneCustomized Vector
- Backbone size w/o insert (bp) 3930
- Total vector size (bp) 5266
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameLyn-R-GECO1
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Insert Size (bp)1336
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GenBank IDJN258411
- Promoter CMV
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site NheI (not destroyed)
- 3′ cloning site NotI (not destroyed)
- 5′ sequencing primer CMV-F
- 3′ sequencing primer SV40-pAR (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
Lyn-R-GECO1 was a gift from Won Do Heo (Addgene plasmid # 120410 ; http://n2t.net/addgene:120410 ; RRID:Addgene_120410) -
For your References section:
Optogenetic toolkit reveals the role of Ca2+ sparklets in coordinated cell migration. Kim JM, Lee M, Kim N, Heo WD. Proc Natl Acad Sci U S A. 2016 May 24;113(21):5952-7. doi: 10.1073/pnas.1518412113. Epub 2016 May 17. 10.1073/pnas.1518412113 PubMed 27190091
Map uploaded by the depositor.
