Fibronectin-human-mutated N-terminal (N), V1 and V2 Glycosylation sites
(Plasmid #120407)

• Purpose: enables eukaryotic expression of human plasma fibronectin in which all three O-glycosylation sites were mutated from threonine to serine
• Depositing Lab: Inaam Nakchbandi
• Publication: Sens et al J Bone Miner Res. 2017 Jan;32(1):70-81. doi: 10.1002/jbmr.2916. Epub 2016 Aug 3.

Backbone

• Vector backbone: pMAX-Cloning
• Backbone size w/o insert (bp): 2868
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: Fibronectin
• Alt name: Oncofetal fibronectin-N-terminal, V1 and V2 mutations
• Alt name: Construct without mutation is referred to as oncofetal fibronectin
• Species: H. sapiens (human)
• Insert Size (bp): 6914
• Mutation: Contains complete variable region with mutations from threonine to serine at position 278/279, 1934 and 1974/1975 at N, V1, and V2 o-Glycosylation sites to prevent O-Glycosylation
• Entrez Gene: FN1 (a.k.a. CIG, ED-B, FINC, FN, FNZ, GFND, GFND2, LETS, MSF, SMDCF)
• Promoter: CMV

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: Unknown (unknown if destroyed)
• 3′ cloning site: Unknown (unknown if destroyed)

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Clone pFN 1.+2.+3. O-Gly_sp_1. It was used to study the role of the various fibronectin isoforms in transfected eucaryotic cells and to produce the isoforms