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Fibronectin-human-EDB in pMAX
(Plasmid #120403)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 120403 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pMAX-Cloning
  • Backbone size w/o insert (bp) 2868
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    EDB-fibronectin
  • Alt name
    Human fibronectin containing complete variable region and EDB (or EIIIB) domain
  • Alt name
    EIIIB fibronectin, mistakingly also called oncofetal fibronectin
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    7187
  • Entrez Gene
    FN1 (a.k.a. CIG, ED-B, FINC, FN, FNZ, GFND, GFND2, LETS, MSF, SMDCF)
  • Promoter CMV

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Unknown (unknown if destroyed)
  • 3′ cloning site Unknown (unknown if destroyed)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Clone EDBpmax_sp_1. It was used to study the role of the various fibronectin isoforms in transfected eucaryotic cells and to produce the isoforms.

See also "Fibronectins containing extradomain A or B enhance osteoblast differentiation via distinct integrins. J Biol Chem. 2017 May 12;292(19):7745-7760." https://www.ncbi.nlm.nih.gov/pubmed/28325836

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Fibronectin-human-EDB in pMAX was a gift from Inaam Nakchbandi (Addgene plasmid # 120403 ; http://n2t.net/addgene:120403 ; RRID:Addgene_120403)

  • For your References section:

    Identification and characterization of a unique role for EDB fibronectin in phagocytosis. Kraft S, Klemis V, Sens C, Lenhard T, Jacobi C, Samstag Y, Wabnitz G, Kirschfink M, Wallich R, Hansch GM, Nakchbandi IA. J Mol Med (Berl). 2016 May;94(5):567-81. doi: 10.1007/s00109-015-1373-0. Epub 2015 Dec 5. 10.1007/s00109-015-1373-0 PubMed 26637426
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