Fibronectin-human-plasma in pMAX
(Plasmid #120401)

• Purpose: enables eukaryotic expression of human plasma fibronectin
• Depositing Lab: Inaam Nakchbandi
• Publication: Rossnagl et al PLoS Biol. 2016 Sep 21;14(9):e1002562. doi: 10.1371/journal.pbio.1002562. eCollection 2016 Sep.

Backbone

• Vector backbone: pMAX-Cloning
• Backbone size w/o insert (bp): 2868
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: Fibronectin
• Alt name: Plasma fibronectin containing complete variable region
• Alt name: Soluble fibronectin
• Species: H. sapiens (human)
• Insert Size (bp): 6914
• Entrez Gene: FN1 (a.k.a. CIG, ED-B, FINC, FN, FNZ, GFND, GFND2, LETS, MSF, SMDCF)
• Promoter: CMV

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: Unknown (unknown if destroyed)
• 3′ cloning site: Unknown (unknown if destroyed)

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Clone pFN sp_1. It was used to study the role of the various fibronectin isoforms in transfected eucaryotic cells and to produce the isoforms.

See also "Fibronectins containing extradomain A or B enhance osteoblast differentiation via distinct integrins. J Biol Chem. 2017 May 12;292(19):7745-7760." https://www.ncbi.nlm.nih.gov/pubmed/28325836