pET42b-ABE7.10
(Plasmid #120398)

• Purpose: Expresses ABE7.10-NLS with an N-terminal His Tag (His6) for bacterial expression
• Depositing Lab: Junjiu Huang
• Publication: Liang et al Nat Commun. 2019 Jan 8;10(1):67. doi: 10.1038/s41467-018-07988-z.

Backbone

• Vector backbone: pET42b
• Backbone size w/o insert (bp): 5248
• Total vector size (bp): 10570
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: ABE7.10
• Alt name: His6-TadA-SGGSSGGS-XTEN-SGGSSGGS-TadA*-SGGSSGGS-XTEN-SGGSSGGS-nCas9-SGGS-NLS
• Species: Synthetic
• Insert Size (bp): 5322
• Mutation: Mammalian codon-optimized
• Promoter: T7
• Tag / Fusion Protein:
  • His (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: Unknown (unknown if destroyed)
• 3′ cloning site: Unknown (unknown if destroyed)
• 5′ sequencing primer: TAATACGACTCACTATAGGG
• 3′ sequencing primer: GCTAGTTATTGCTCAGCGG

Resource Information

• Supplemental Documents:
  • pET42b-ABE7.10.gb
• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry