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Addgene

pGEM4Z globin NB Probe
(Plasmid #120390)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 120390 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pGEM4Z
  • Backbone manufacturer
    Promega
  • Backbone size w/o insert (bp) 2746
  • Total vector size (bp) 2932
  • Vector type
    Unspecified

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    beta globin partial 3' UTR
  • Alt name
    HBB 3 'UTR
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site XmaI (not destroyed)
  • 3′ cloning site BamHI (not destroyed)
  • 5′ sequencing primer GTTTTCCCAGTCACGAC
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pGEM4Z globin NB Probe was a gift from Niels Gehring (Addgene plasmid # 120390 ; http://n2t.net/addgene:120390 ; RRID:Addgene_120390)

  • For your References section:

    Detection and quantification of RNA decay intermediates using XRN1-resistant reporter transcripts. Voigt F, Gerbracht JV, Boehm V, Horvathova I, Eglinger J, Chao JA, Gehring NH. Nat Protoc. 2019 May;14(5):1603-1633. doi: 10.1038/s41596-019-0152-8. Epub 2019 Apr 24. 10.1038/s41596-019-0152-8 PubMed 31019309
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