pMAL-PUM2
(Plasmid #120385)

• Purpose: Bacterial expression of MBP-Pumillio2 fusion protein
• Depositing Lab: Igor Ulitsky
• Publication: Tichon et al Nat Commun. 2016 Jul 13;7:12209. doi: 10.1038/ncomms12209.

Backbone

• Vector backbone: pMAL-C1
• Total vector size (bp): 9578
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): Rosetta3
• Growth instructions: Chloramfenicol resistance in bacterial strain 50µg/ml
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: PUM2
• Species: H. sapiens (human)
• Insert Size (bp): 2955
• GenBank ID: NM_001282791 NP_001269720
• Entrez Gene: PUM2 (a.k.a. PUMH2, PUML2)
• Promoter: LacI
• Tags / Fusion Proteins:
  • MBP (N terminal on backbone)
  • 6xHIS (N terminal on backbone)

Cloning Information

• Cloning method: Ligation Independent Cloning
• 5′ sequencing primer: GAAGCCCTGAAAGACGCG
• 3′ sequencing primer: CCTCTTCGCTATTACGCC

Resource Information

• Supplemental Documents:
  • pMAL-PUM2.gbk
• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Please note that mutations G518V and L520R in PUM2 [NM_001282791] were found during Addgene's quality control process. The depositor has noted these mutations should NOT affect plasmid function.