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pMAL-PUM1
(Plasmid #120384)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 120384 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pMAL-C1
  • Total vector size (bp) 10187
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    Rosetta3
  • Growth instructions
    Chloramphenicol resistance in growth strain 50µg/ml
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    PUM1
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    3564
  • GenBank ID
    NM_001020658 NP_001018494
  • Entrez Gene
    PUM1 (a.k.a. HSPUM, NEDMSF, PUMH, PUMH1, PUML1, SCA47)
  • Promoter LacI
  • Tags / Fusion Proteins
    • MBP (N terminal on backbone)
    • 6xHIS (N terminal on backbone)

Cloning Information

  • Cloning method Ligation Independent Cloning
  • 5′ sequencing primer GAAGCCCTGAAAGACGCG
  • 3′ sequencing primer CCTCTTCGCTATTACGCC
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pMAL-PUM1 was a gift from Igor Ulitsky (Addgene plasmid # 120384 ; http://n2t.net/addgene:120384 ; RRID:Addgene_120384)

  • For your References section:

    A conserved abundant cytoplasmic long noncoding RNA modulates repression by Pumilio proteins in human cells. Tichon A, Gil N, Lubelsky Y, Havkin Solomon T, Lemze D, Itzkovitz S, Stern-Ginossar N, Ulitsky I. Nat Commun. 2016 Jul 13;7:12209. doi: 10.1038/ncomms12209. 10.1038/ncomms12209 PubMed 27406171
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