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PurposeExpression vector for lncRNA NORAD
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 120383 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepcDNA3.1
- Total vector size (bp) 11147
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Vector typeMammalian Expression
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature30°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameNORAD
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Alt nameLINC00657
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SpeciesH. sapiens (human)
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GenBank IDNR_027451
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Entrez GeneNORAD (a.k.a. LINC00657)
- Promoter CMV
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer T7 (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pcDNA3.1-NORAD was a gift from Igor Ulitsky (Addgene plasmid # 120383 ; http://n2t.net/addgene:120383 ; RRID:Addgene_120383) -
For your References section:
A conserved abundant cytoplasmic long noncoding RNA modulates repression by Pumilio proteins in human cells. Tichon A, Gil N, Lubelsky Y, Havkin Solomon T, Lemze D, Itzkovitz S, Stern-Ginossar N, Ulitsky I. Nat Commun. 2016 Jul 13;7:12209. doi: 10.1038/ncomms12209. 10.1038/ncomms12209 PubMed 27406171