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Addgene

pAcGFP-MALAT1M
(Plasmid #120382)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 120382 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pAcGFP1-C1
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    MALAT1
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    625
  • GenBank ID
    NR_002819
  • Entrez Gene
    MALAT1 (a.k.a. HCN, LINC00047, NCRNA00047, NEAT2, PRO2853)
  • Promoter CMV
  • Tag / Fusion Protein
    • AcGFP1 (N terminal on insert)

Cloning Information

  • Cloning method Ligation Independent Cloning
  • 5′ sequencing primer acgagaagcgcgatcacatg
  • 3′ sequencing primer gtgatgctattgctttatttgtaacc
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pAcGFP-MALAT1M was a gift from Igor Ulitsky (Addgene plasmid # 120382 ; http://n2t.net/addgene:120382 ; RRID:Addgene_120382)

  • For your References section:

    Sequences enriched in Alu repeats drive nuclear localization of long RNAs in human cells. Lubelsky Y, Ulitsky I. Nature. 2018 Mar 1;555(7694):107-111. doi: 10.1038/nature25757. Epub 2018 Jan 24. 10.1038/nature25757 PubMed 29466324
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