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Addgene

pET-3a-His-MBP-TEV-VC_Cas1_WT
(Plasmid #120364)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 120364 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pET3a
  • Backbone size w/o insert (bp) 5969
  • Total vector size (bp) 7094
  • Vector type
    Bacterial Expression, CRISPR

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    VC_Cas1
  • Insert Size (bp)
    1125

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pET-3a-His-MBP-TEV-VC_Cas1_WT was a gift from Jennifer Doudna (Addgene plasmid # 120364 ; http://n2t.net/addgene:120364 ; RRID:Addgene_120364)

  • For your References section:

    A Functional Mini-Integrase in a Two-Protein-type V-C CRISPR System. Wright AV, Wang JY, Burstein D, Harrington LB, Paez-Espino D, Kyrpides NC, Iavarone AT, Banfield JF, Doudna JA. Mol Cell. 2019 Feb 21;73(4):727-737.e3. doi: 10.1016/j.molcel.2018.12.015. Epub 2019 Jan 29. 10.1016/j.molcel.2018.12.015 PubMed 30709710
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