pMOD3-mNeptune2-nat
(Plasmid
#120335)
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PurposeBacterial mNeptune2 promoter trap
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 120335 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepMOD3
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Backbone manufacturerEpicentre
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Vector typeBacterial Expression
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Selectable markersNourseothricin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB 5-alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namemNeptune2
- Promoter (None)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site EcoRI (unknown if destroyed)
- 3′ cloning site HindIII (unknown if destroyed)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pMOD3-mNeptune2-nat was a gift from James Kirby (Addgene plasmid # 120335 ; http://n2t.net/addgene:120335 ; RRID:Addgene_120335) -
For your References section:
Promotion and Rescue of Intracellular Brucella neotomae Replication during Coinfection with Legionella pneumophila. Kang YS, Kirby JE. Infect Immun. 2017 Apr 21;85(5). pii: e00991-16. doi: 10.1128/IAI.00991-16. Print 2017 May. 10.1128/IAI.00991-16 PubMed 28264909