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Addgene

pAT28-proD/SaEGFP
(Plasmid #120321)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 120321 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pAT28
  • Backbone manufacturer
    Patrick Trieu-Cuot
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Spectinomycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB 5-alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    eGFP with Staphylococcus aureus codon optimization
  • Species
    Synthetic
  • Promoter proD

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (unknown if destroyed)
  • 3′ cloning site SalI (unknown if destroyed)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pAT28-proD/SaEGFP was a gift from James Kirby (Addgene plasmid # 120321 ; http://n2t.net/addgene:120321 ; RRID:Addgene_120321)

  • For your References section:

    Promotion and Rescue of Intracellular Brucella neotomae Replication during Coinfection with Legionella pneumophila. Kang YS, Kirby JE. Infect Immun. 2017 Apr 21;85(5). pii: e00991-16. doi: 10.1128/IAI.00991-16. Print 2017 May. 10.1128/IAI.00991-16 PubMed 28264909
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