AAV_hSyn_Ilmol
(Plasmid #120311)

• Purpose: Expresssion of Ilmol preferentially in neurons
• Depositing Lab: Bradley Baker
• Publication: Yi et al Sci Rep. 2018 Oct 12;8(1):15199. doi: 10.1038/s41598-018-33297-y.

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Backbone

• Vector backbone: pAAV
• Backbone size w/o insert (bp): 4500
• Total vector size (bp): 6000
• Vector type: Mammalian Expression, AAV

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Ilmol
• Alt name: Ilmol
• Promoter: hSyn

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NheI (not destroyed)
• 3′ cloning site: EcoRV (not destroyed)
• 5′ sequencing primer: CCC AAG CTG GCT AGC GCC ACC ATG GAG GGA TTC GAC
• 3′ sequencing primer: GAT AAG CTT GAT ATC GAA TTC TCA CTT GTA CAG CTC GTC

Resource Information

• Supplemental Documents:
  • pAAV-hSyn-ilmol.gbk

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry