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Addgene

AAV_hSyn_Ilmol
(Plasmid #120311)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 120311 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pAAV
  • Backbone size w/o insert (bp) 4500
  • Total vector size (bp) 6000
  • Vector type
    Mammalian Expression, AAV

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Ilmol
  • Alt name
    Ilmol
  • Promoter hSyn

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NheI (not destroyed)
  • 3′ cloning site EcoRV (not destroyed)
  • 5′ sequencing primer CCC AAG CTG GCT AGC GCC ACC ATG GAG GGA TTC GAC
  • 3′ sequencing primer GAT AAG CTT GAT ATC GAA TTC TCA CTT GTA CAG CTC GTC
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    AAV_hSyn_Ilmol was a gift from Bradley Baker (Addgene plasmid # 120311 ; http://n2t.net/addgene:120311 ; RRID:Addgene_120311)

  • For your References section:

    A dimeric fluorescent protein yields a bright, red-shifted GEVI capable of population signals in brain slice. Yi B, Kang BE, Lee S, Braubach S, Baker BJ. Sci Rep. 2018 Oct 12;8(1):15199. doi: 10.1038/s41598-018-33297-y. 10.1038/s41598-018-33297-y [pii] PubMed 30315245
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