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Addgene

Ilmol
(Plasmid #120310)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 120310 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pcDNA3.1
  • Backbone size w/o insert (bp) 5500
  • Total vector size (bp) 6900
  • Vector type
    Mammalian Expression
  • Selectable markers
    Hygromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Ilmol
  • Alt name
    Ilmol
  • Species
    Synthetic
  • Insert Size (bp)
    1474
  • Promoter CMV

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NheI (not destroyed)
  • 3′ cloning site XhoI (not destroyed)
  • 5′ sequencing primer CMV-forward
  • 3′ sequencing primer BGH-R
  • (Common Sequencing Primers)

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Ilmol was a gift from Bradley Baker (Addgene plasmid # 120310 ; http://n2t.net/addgene:120310 ; RRID:Addgene_120310)
  • For your References section:

    A dimeric fluorescent protein yields a bright, red-shifted GEVI capable of population signals in brain slice. Yi B, Kang BE, Lee S, Braubach S, Baker BJ. Sci Rep. 2018 Oct 12;8(1):15199. doi: 10.1038/s41598-018-33297-y. 10.1038/s41598-018-33297-y [pii] PubMed 30315245