Ilmol
(Plasmid
#120310)
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PurposeA red-shifted GEVI consists of triple mutant ciona based voltage sensing domain with optimized linker and dTomato K159R.
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 120310 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepcDNA3.1
- Backbone size w/o insert (bp) 5500
- Total vector size (bp) 6900
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Vector typeMammalian Expression
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Selectable markersHygromycin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameIlmol
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Alt nameIlmol
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SpeciesSynthetic
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Insert Size (bp)1474
- Promoter CMV
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site NheI (not destroyed)
- 3′ cloning site XhoI (not destroyed)
- 5′ sequencing primer CMV-forward
- 3′ sequencing primer BGH-R (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
Ilmol was a gift from Bradley Baker (Addgene plasmid # 120310 ; http://n2t.net/addgene:120310 ; RRID:Addgene_120310) -
For your References section:
A dimeric fluorescent protein yields a bright, red-shifted GEVI capable of population signals in brain slice. Yi B, Kang BE, Lee S, Braubach S, Baker BJ. Sci Rep. 2018 Oct 12;8(1):15199. doi: 10.1038/s41598-018-33297-y. 10.1038/s41598-018-33297-y [pii] PubMed 30315245