AAV CMV-driven AcrIIC1-scaffold (2xBsmBI sites)
(Plasmid
#120300)
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PurposeAAV vector for expression of AcrIIC1 (no miR binding sites, control vector)
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 120300 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonescAAV
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Vector typeMammalian Expression, AAV, CRISPR
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameAcrIIC1
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Alt nameU6 scaffold
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site Unknown (unknown if destroyed)
- 3′ cloning site Unknown (unknown if destroyed)
Resource Information
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Supplemental Documents
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please visit https://www.biorxiv.org/content/10.1101/480384v1 for BioRxiv preprint
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
AAV CMV-driven AcrIIC1-scaffold (2xBsmBI sites) was a gift from Dominik Niopek (Addgene plasmid # 120300 ; http://n2t.net/addgene:120300 ; RRID:Addgene_120300) -
For your References section:
Cell-specific CRISPR-Cas9 activation by microRNA-dependent expression of anti-CRISPR proteins. Hoffmann MD, Aschenbrenner S, Grosse S, Rapti K, Domenger C, Fakhiri J, Mastel M, Borner K, Eils R, Grimm D, Niopek D. Nucleic Acids Res. 2019 Apr 15. pii: 5458120. doi: 10.1093/nar/gkz271. 10.1093/nar/gkz271 PubMed 30982889